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目的:探讨Toll样受体4(Toll like receptor 4,TLR4)在滋养细胞中的表达及其免疫作用。方法:采用RT-PCR分别检测原代培养滋养细胞和经1mg/LLPS刺激12h后的滋养细胞中TLR4 mRNA表达水平;流式细胞计数分别检测LPS刺激的滋养细胞中TLR4抗体封闭组、非TLR4抗体封闭组和空白对照组细胞凋亡发生率;ELISA法分别检测LPS刺激的滋养细胞中TLR4抗体封闭组、非TLR4抗体封闭组和空白对照组细胞TNF-α分泌水平。结果:RT-PCR结果显示,经LPS刺激的滋养细胞较正常滋养细胞TLR4mRNA表达水平明显增强;在LPS刺激的滋养细胞中,流式细胞计数结果显示,TLR4抗体封闭组和非TLR4抗体封闭组细胞凋亡率存在明显差异。ELISA结果显示,TLR4抗体封闭组和非TLR4抗体封闭组细胞TNF-α分泌水平也有显著差异。结论:TLR4信号途径在LPS引起的人滋养细胞凋亡及TNF-α分泌等炎性过程中发挥着重要作用。
Objective: To investigate the expression of Toll like receptor 4 (TLR4) in trophoblast cells and its immune function. Methods: The expression of TLR4 mRNA in primary cultured trophoblast cells and trophoblast cells stimulated by 1mg / LLPS for 12h were detected by RT-PCR. Flow cytometry was used to detect TLR4 antibody-blocked group and non-TLR4 antibody Blocking group and blank control group. The levels of TNF-α in TLR4-blocking group, non-TLR4 blocking group and blank control group were detected by ELISA. Results: RT-PCR results showed that the expression level of TLR4 mRNA in trophoblast cells stimulated by LPS was significantly higher than that in normal trophoblast cells. Flowcytometry in trophoblasts stimulated by LPS showed that TLR4-blocking group and non-TLR4 blocking group cells There is a significant difference in apoptotic rate. The result of ELISA showed that TNF-α secretion of TLR4-blocking group and non-TLR4 blocking group also had significant difference. Conclusion: The TLR4 signaling pathway plays an important role in the inflammatory processes such as apoptosis of human trophoblast and secretion of TNF-α induced by LPS.