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目的研究吖啶橙-溴酸钾-萘酚体系的光谱性质及其共振光散射增强机制,建立共振光散射法测定人尿中痕量萘酚新方法。方法在稀硫酸介质中,萘酚与溴酸钾、吖啶橙(acridine orange,AO)发生反应形成聚集离子,导致共振光散射(resonance light scattering,RLS)显著增强,产生新的RLS光谱。研究了反应体系的吸收、荧光、RLS光谱特征,适宜的反应条件。结果α-萘酚和β-萘酚最大的RLS峰均位于468.0 nm处,线性范围分别为1.41×10-7～2.80×10-5 mol/L,1.28×10-7～3.00×10-5 mol/L,相关系数分别为0.999 6,0.999 3,检出限分别为0.422×10-7 mol/L和0.385×10-7 mol/L。RSD为4.8%～7.3%,平均回收率为90.7%(n=6)。结论该方法灵敏度高,便捷,成本低,可用于检测人尿中的痕量α-萘酚,结果满意。
Aim To study the spectroscopic properties of acridine orange-potassium bromate-naphthol system and its enhancement mechanism of resonance light scattering (RIA), a new method for the determination of trace naphthol in human urine by resonance light scattering was established. Methods In dilute sulfuric acid medium, naphthol reacts with potassium bromate and acridine orange (AO) to form aggregated ions, resulting in a significant increase of resonance light scattering (RLS), resulting in a new RLS spectrum. The absorption, fluorescence and RLS spectra of the reaction system were studied. The suitable reaction conditions were studied. Results The maximum RLS peaks of α-naphthol and β-naphthol were all at 468.0 nm with linear ranges of 1.41 × 10-7 ~ 2.80 × 10-5 mol / L, 1.28 × 10-7 ~ 3.00 × 10-5 mol / L, respectively. The correlation coefficients were 0.999 6 and 0.999 3, respectively. The detection limits were 0.422 × 10-7 mol / L and 0.385 × 10-7 mol / L, respectively. RSD was 4.8% ~ 7.3%, the average recovery was 90.7% (n = 6). Conclusion The method is sensitive, convenient and low cost and can be used for the determination of trace α-naphthol in human urine with satisfactory results.