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AIM:To study the effects of sense and antisense KAI1genes on the growth and invasion of human hepatocellularcarcinoma (HCC) cell line MHCC97-H.METHODS:KAI1 sense and antisense eukaryoticexpression plasmids were constructed using subclonetechnique and transfected into MHCC97-H cells respectivelyby DOTAP liposome.After successful transfection wasconfirmed,in vitro growth curve,cell cycles,plate cloneformation efficiency,invasive ability in Boyden Chamberassay and ultrastructural morphology were studied.RESULTS:KAI1 sense and antisense genes had nosignificant effects on the cell growth curve and cell cycles.After transfection with sense KAI1 gene,decreased invasiveability in Boyden Chamber assay and decreased amount ofmitochondria,but no significant changes of plate cloneformation efficiency were observed in MHCC97-H-S cells.The plate clone formation efficiency and invasive ability inBoyden Chamber assay were significantly increased inMHCC97-H-AS cells,after transfection with antisense KAI1gene.Furthermore,increased amount of mitochondria,rough endoplasmic reticulum,Golgi apparatus andexpanded endoplasmic reticulum were also noted inMHCC97-H-AS cells.CONCLUSION:Changes of KAI1 expression in HCC cellsmay alter their invasive and metastasis ability of the tumor.
AIM: To study the effects of sense and antisense KAI1 genes on the growth and invasion of human hepatocellular carcinoma (HCC) cell line MHCC97-H. METHODS: KAI1 sense and antisense eukaryoticexpression plasmids were constructed using subclonetechnique and transfected into MHCC97-H cells respectively by DOTAP liposome . After successful transfection wasconfirmed, in vitro growth curve, cell cycles, plate cloneformation efficiency, invasive ability in Boyden Chamberassay and ultrastructural morphology were studied .RESULTS: KAI sense and antisense genes had nosignificant effects on the cell growth curve and cell cycles. After transfection with sense KAI gene, decreased invasive ability in Boyden Chamber assay and decreased amount of mitochondria, but no significant changes of plate clone formation efficiency efficiency observed in MHCC97-HS cells. The plate clone formation efficiency and invasive ability in Boyden Chamber assay were significantly increased in MHCC97-H- AS cells, after transfection with antisense KA I1 gene.Furthermore, increased amount of mitochondria, rough endoplasmic reticulum, Golgi apparatus andexpanded endoplasmic reticulum were also noted in MHCC97-H-AS cells. CONCLUSION: Changes of KAI1 expression in HCC cells may alter their invasive and metastasis ability of the tumor.