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AIM:To study interactions between hepatitis B virus(HBV)and interferon-α in liver-derived cells.METHODS:mRNAs were separately isolated from an HBV-transfected cell line(HepG_22.2.15)and its parental cell line(HepG_2)pre- and post-interferon-α(IFN-α)treatment at 6,24 and 48h,followed by hybridization with a cDNA microarrayfilter dotted with 14000 human genes.After hybridizationand scanning of the arrays,the data were analyzed usingArrayGauge software.The microarray data were furtherverified by Northern blot analysis.RESULTS:Compared to HepG_2 cells,14 genes with knownfunctions were down-regulated 3 to 12-magnitudes,while 7genes were up-regulated 3-13 magnitudes in HepG_22.2.15cells prior to IFN-α treatment.After interferon-α treatment,the expression of four genes(vascular endothelial growthfactor,tyrosine phosphate 1E,serine protein with IGF-bindingmotif and one gene of clathrin light chain)in HepG_22.2.15were up-regulated,while one gene encoding a GTP-bindingprotein,two genes of interferon-induced kinases and twoproto-oncogenes were further down- regulated.Interestingly,under IFN-α treatment,a number of differentially regulatedgenes were new ESTs or genes with unknown functions.CONCLUSION:The up-regulated genes in HepG_22.2.15cell line suggested that under IFN-α treatment,theserepressed cellular genes in HBV infected hepatocytes couldbe partially restored,while the down-regulated genes weremost likely the cellular genes which could not be restoredunder interferon treatment.These down-regulated genesidentified by microarray analysis could serve as new targetsfor anti-HBV drug development or for novel therapies.
AIM: To study interactions between hepatitis B virus (HBV) and interferon-α in liver-derived cells. METHODS: mRNAs were isolated isolated from an HBV-transfected cell line (HepG_22.2.15) and its parental cell line (HepG_2) and post-interferon-α (IFN-α) treatment at 6, 24 and 48h, followed by hybridization with a cDNA microarray filipter dotted with 14000 human genes. After hybridization and scanning of the arrays, the data were analyzed using ArrayGauge software. microarray data were furtherverified by Northern blot analysis .RESULTS: Compared to HepG_2 cells, 14 genes with knownfunctions were down-regulated 3 to 12-magnitudes, while 7genes were up-regulated 3-13 magnitudes in HepG_22.2.15cells prior to IFN-α treatment. After interferon-α treatment, the expression of four genes (vascular endothelial growth factor, tyrosine phosphate 1E, serine protein with IGF-binding motif and one gene of clathrin light chain) in HepG_22.2.15were up-regulated, while one gene encoding a GTP-bindingprotein two genes of i nterferon-induced kinases and twoproto-oncogenes were further down-regulated.Interestingly, under IFN-α treatment, a number of differentially regulated genes were new ESTs or genes with unknown functions. CONCLUSION: The up-regulated genes in HepG_22.2.15 cell line suggested that under IFN-α treatment, theserepressed cellular genes in HBV infected hepatocytes could be partially restored, while the down-regulated genes were most likely the cellular genes which could not be restoredunder interferon treatment.These down-regulated genesesidentified by microarray analysis could serve as new targetsfor anti-HBV drug development or for novel therapies.