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选用15个采自新疆不同地区的西瓜枯萎病菌株,在含KClO3培养基上诱导出抗氯酸盐的不能利用硝酸盐营养体突变株(nitmutant),在不同氮源培养基上划分出nit1,nit2,nit8,nitM和nitX5种突变类型,其中nit1出现频率最高。将各菌株的nit突变株和测试菌株的nitM或nit8配对,结果15个菌株分为6个不同的营养体亲和群(VCGs),其中1号小种的菌株划为VCG1亲和群,0号小种划为VCG2,2株非致病菌株分别为VCG3和VCG4,2株只出现nitX突变类型的菌株分别为VCG5和VCG6。表明营养体亲和群与生理小种存在一致关系,营养体亲和性可以作为西瓜枯萎病菌生理小种鉴定和病原菌种群遗传结构研究的有效手段之一。
A total of 15 strains of Fusarium oxysporum f. Sp. From different regions of Xinjiang were selected to induce nitrification of nitrate resistant mutants on KClO3 medium, nit2, nit8, nitM and nitX5 types of mutations, including nit1 highest frequency. The strains of nit mutants of each strain were paired with nitM or nit8 of the test strains. As a result, 15 strains were divided into 6 different vegetative affinity groups (VCGs), in which strain No. 1 was classified as VCG1 affinity group and 0 Number of races were classified as VCG2, 2 strains of non-pathogenic strains were VCG3 and VCG4, 2 strains only appeared nitX mutation types were VCG5 and VCG6. The results showed that there was a consistent relationship between vegetative affinity groups and physiological races, and nutrient body affinity could be used as one of the effective methods to identify the physiological races of Fusarium oxysporum f.