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癌细胞机械力学特性的测量为癌症的诊断、预防、治疗、病变机理等方面的研究提供全新的手段和巨大的潜在空间.本文建立细胞和针尖之间的接触压力和针尖在细胞上切入深度的计算模型,利用原子力显微镜(AFM)单晶硅针尖对固定的卵巢癌细胞(UACC1598)和结肠癌细胞(NCI-H716)进行微切削逐层去除.结果表明:由于不同细胞的结构和机械性质的差异,相同的加载力对不同的细胞材料进行切削加工时,细胞和针尖之间的接触压力和针尖在细胞上的切入深度差异较大.细胞表面微切削去除范围为8m×8m,在细胞上逐层去除材料时的加载力从17.523到32.126N逐渐增大,去除后对每层不同位置进行弹性模量测量,得到细胞内部弹性模量分布,细胞内部弹性模量与细胞表面弹性模量差分别为卵巢癌细胞0.288±0.08kPa,结肠癌细胞0.376±0.16kPa.用这种微切削去除方法可以测量细胞内部细胞骨架细胞器等各结构的力学特性,为疾病的诊断和治疗提供更精确的实验数据.
Measurement of the mechanical properties of cancer cells provides a new means and a huge potential space for the research of cancer diagnosis, prevention, treatment, pathology, etc. This article establishes the contact pressure between the cells and the tip and the penetration depth of the tip The model was calculated and the micro-ablation of fixed ovarian cancer cells (UACC1598) and colon cancer cells (NCI-H716) was carried out by AFM single-crystal silicon tips.The results showed that due to the different structure and mechanical properties of different cells Difference, the same loading force on different cell materials for cutting, the contact pressure between the cell and the tip and the depth of the tip on the cell depth of the larger micro-cutting removal of the cell surface in the range of 8m × 8m, on the cells The loading force of layer-by-layer material removal increased gradually from 17.523 to 32.126N. After removal, the elastic modulus of each layer was measured to get the distribution of internal elastic modulus, the difference between the internal elastic modulus and the cell surface elastic modulus Respectively ovarian cancer cells 0.288 ± 0.08kPa, colon cancer cells 0.376 ± 0.16kPa. With this method of micro-cutting can be measured inside the cell cytoskeleton Organelles and other mechanical properties of the structure for the diagnosis and treatment of disease to provide more accurate experimental data.