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为了确定连锁图上着丝点的位置，常常需要一些特殊的遗传材料，如端部着丝点染色体和等臂染色体材料等等。在水稻上，为了获得这类遗传材料，从中籼３０３７同源三倍体后代产生的非整倍体中，在筛选初级三体的同时，进行了等臂染色体变异材料的筛选。其中在三体１０的后代中发现了１株形态与三体１０相似的变异株，经细胞学检查，证明它是第十染色体短臂的等臂四体。利用该等臂四体及其自交后代中分离出的次级三体，以及相应染色体的初级三体和正常二倍体为材料，通过ＲＦＬＰ不同分子标记在这些材料上所表现剂量效应的不同，确定Ｇ１１２５、Ｇ３３３、Ｌ１６９等３个分子标记位于第十染色体短臂上，Ｇ１０８４及其它１６个分子标记位于第十染色体长臂上。参照Ｋｕｒａｔａ等的分子图谱，将第十染色体的着丝点定位在分子标记Ｇ１１２５和Ｇ１０８４之间，两标记相距３．２ｃＭ［８］。
In order to determine the position of the centromere on the linkage map, it is often necessary to have some special genetic material, such as the end centromere and isobaric chromosome material and so on. In rice, in order to obtain this kind of genetic material, from the aneuploidy produced by the progeny of Zhong373037, we screened the primary trisomy and conducted the screening of isobar heterozygotes. In the offspring of trisomy 10, a mutant strain with a similar morphology to that of trisomy 10 was found. It was proved by cytology that it was the tetragon of the short arm of chromosome 10. Using the secondary trisomy isolated from the tetraploid and its selfing offspring, as well as the primary trisomy and normal diploid of the corresponding chromosome, different doses of RFLP showed different dose effects on these materials The three molecular markers G1125, G333 and L169 were located on the short arm of chromosome 10, and the G1084 and other 16 molecular markers were located on the long arm of chromosome ten. According to Kurata et al. Molecular mapping, the centromere of chromosome 10 was located between molecular markers G1125 and G1084, and the distances between the two markers were 3.2 cM [8].