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神经脑化学研究,要求测量动物死后所欲测物质含量死前瞬间的实际值。但由于动物死后脑酶系统并未停止活动,因而欲测物质含量往往与死前瞬间实际值不符而造成伪差。故动物死后迅速停止酶系统的活性便成为脑化学研究工作中急待解决的问题。 历史上最先采用的技术是冷冻法。此法是将动物(如大鼠)离断的头部浸泡在液氮或氟里昂-12中,以达到使酶失活。冷冻法原理是基于脑组织的热传导特性,因而使脑区全部冷却往往长达90秒钟以上,这
Neuronal brain chemistry requires the measurement of the actual value of the moment immediately before the death of the animal at which the substance is to be measured. However, due to the death of animal brain enzyme system did not stop the activity, and thus to test the substance content is often inconsistent with the actual value of the moment before death caused by artificial error. Therefore, the animal’s rapid stop after the death of the enzyme system activity has become an urgent problem in brain chemistry research. The first technology used in history was freezing. This method is the animals (such as rats) cut off the head soaked in liquid nitrogen or Freon -12 in order to achieve the enzyme inactivation. The principle of freezing is based on the heat conduction characteristics of the brain tissue, so the total cooling of the brain area is often as long as 90 seconds, which