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目的：测定致倦库蚊幼虫、具抗性蚊幼虫可溶性蛋白的组分，并分析、评价其特异性蛋白区带。方法：对正常和具抗性的Ⅲ龄、Ⅳ龄初幼虫的可溶性蛋白提取物进行聚丙烯酰胺凝胶电泳分析。结果：正常蚊幼虫主要有１２条蛋白区带，其中５５ＫＤａ和５０ＫＤａ为其特有；对马拉硫磷及溴氰菊酯具抗性的蚊幼虫，分别有１５和１７条蛋白区带，并发现６６ＫＤａ蛋白为特异性蛋白分子。结论：具抗性的蚊幼虫所特有的６６ＫＤａ蛋白分子可能为导致蚊幼虫对杀虫剂产生抗性的特异性蛋白。
Objective: To determine the components of soluble protein of Larvae of Culex pipiens quinquefasciatus, resistant mosquito mosquito, and to analyze and evaluate the specific protein bands. Methods: Polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze the soluble protein extracts of normal and resistant early stage III and IV larvae. Results: Normal mosquito larvae mainly had 12 protein bands, of which 55 KDa and 50 KDa were endemic. Mosquito larvae resistant to malathion and deltamethrin had 15 and 17 protein bands, respectively, and found that The 66 kDa protein is a specific protein molecule. CONCLUSION: The 66KDa protein molecule, which is unique to resistant mosquito larvae, may be a specific protein that causes mosquito larvae to develop resistance to insecticides.