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本文旨在用定量方法比较健康产妇与子宫内膜炎产妇子宫腔的细菌丛和研究这些分离菌对抗生素的敏感性.受检者为产后子宫内膜炎产妇26例(第一组)和产后正常的产妇10例(第二组).子宫腔分泌物细菌培养,第一组于子宫内膜炎症状出现至应用抗生素之前,第二组始于产后第3～4天.需氧菌培养,将试样接种于5%血液琼脂固体培养基和含1%糖肉汤液体培养基,37℃下培养18小时.为获得厌氧菌和微需氧菌,将试样接种于加入5%正常人血液、5%溶解人血液和1%氯高铁血红素液的心-脑琼脂,以及培养类杆菌的半固体培养基,在80%氮、10%氢和10%二氧化碳的严格无氧
The purpose of this paper is to compare quantitatively the bacterial flora of the uterine cavity of healthy maternal and endometritis women and to study the sensitivity of these isolates to antibiotics.The subjects were postpartum 26 cases of postpartum endometritis maternal (first group) 10 cases of normal maternal (second group). Bacterial culture of uterine cavity secretions, the first group appeared in the symptoms of endometritis before the application of antibiotics, the second group began after 3 to 4 days postpartum. Aerobic culture, The samples were inoculated on 5% blood agar solid medium and 1% sugar broth liquid medium and cultured for 18 hours at 37 ° C. To obtain anaerobic bacteria and micro-aerobic bacteria, the samples were inoculated into 5% normal Human brain blood, 5% lysed human blood, and 1% hemin-agar, as well as semi-solid culture medium for culturing Bacteroides, were strictly oxygen-free at 80% nitrogen, 10% hydrogen and 10% carbon dioxide