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目的通过研究锰暴露人群外周铁代谢的变化,探讨锰暴露对作业工人外周铁代谢的影响。方法全部实验对象均来自北京同一单位,暴露组为从事手工电弧焊作业的97名工人(使用焊条为J422型),对照组为非有害作业工人91名;按照国家标准规定的方法对锰暴露者工作场所空气中的锰浓度进行了采样分析;采用石墨炉原子吸收法检测血清锰、血清铁,应用双抗夹心酶联免疫的方法检测血清铁蛋白、转铁蛋白、转铁蛋白受体的浓度。结果 3个锰暴露工作场所空气中锰的 PC-STEL范围是0．53-2．19 mg／m3,PC-TWA范围是0．29～0．92 mg/m3。暴露组血清转铁蛋白、血清铁、血清锰浓度均高于对照组,差异有统计学意义(P<0．05,P<0．01,P<0．01),而暴露组血清转铁蛋白受体明显低于对照组(P=0．01),虽然血清铁蛋白水平两组比较差异无统计学意义(P>0．05), 但暴露组铁蛋白水平有升高的趋势;暴露组血清铁和血清锰异常率分别是55．67％和67．01％,与对照组异常率比较差异有统计学意义,且暴露组血清转铁蛋白含量异常率也有升高的趋势。单因素方差分析显示,各项指标的变化趋势随着锰暴露工龄的增加而变化,未发现各生物指标与锰暴露时间的线性相关关系(P>0．05)。结论长期锰暴露可导致铁代谢的失衡,表现在血清铁、转铁蛋白的增高和血清转铁蛋白受体的下降。
Objective To study the effects of manganese exposure on peripheral iron metabolism in workers exposed to manganese by studying the changes of peripheral iron metabolism in the exposed population of manganese. Methods All the subjects were from the same unit in Beijing. The exposure group was 97 workers engaged in manual arc welding (using electrode J422), and 91 non-hazardous workers in the control group. In the control group, The concentration of Mn in workplace air was sampled and analyzed. The levels of serum ferritin, transferrin and transferrin receptor were detected by graphite furnace atomic absorption spectrometry after serum manganese and serum iron were detected by double antibody sandwich enzyme-linked immunosorbent assay . Results The PC-STEL range of manganese in the air of the three manganese exposure sites was 0.53-2.19 mg / m3 and the PC-TWA range was 0.29-0.92 mg / m3. The levels of serum transferrin, serum iron and serum manganese in the exposed group were significantly higher than those in the control group (P <0.05, P <0.01, P <0.01) (P = 0.01). Although the level of serum ferritin was no significant difference between the two groups (P> 0.05), the level of ferritin in the exposed group had a tendency to increase; The serum iron and serum manganese abnormalities were 55.67% and 67.01% respectively, which were significantly different from those in the control group, and the abnormal rates of serum transferrin in the exposed group were also increased. Univariate analysis of variance showed that the trend of each index changed with the increase of the service life of manganese exposure. No linear correlation was found between each index and the exposure time of manganese (P> 0.05). Conclusion Long-term exposure to manganese can lead to an imbalance of iron metabolism, manifested in increased serum iron, transferrin and decreased serum transferrin receptor.