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目的探讨血管内皮损伤与铁调素(hepcidin)高表达的相关性,以及川芎嗪对其的干预作用。方法 24只SD大鼠随机分为4组:对照组、模型组、肝素阳性对照组(肝素组)及川芎嗪组,每组6只。对照组大鼠以普通饲料喂养,其余3组以高脂饲料喂养。8周后,对照组及模型组大鼠ip给予生理盐水2mL/kg,肝素组ip给予肝素钠注射液5mg/kg,川芎嗪组ip给予盐酸川芎嗪注射液40mg/kg,均连续给药7d。大鼠腹主动脉取血后分离血清,检测各组大鼠血脂指标,检测血清铁调素、一氧化氮(NO)、内皮素1(ET-1)、活性氧(ROS)、丙二醛(MDA)、过氧化氢酶(CAT)及超氧化物歧化酶(SOD)水平;截取主动脉组织,进行铁含量检测及组织病理学观察。结果与对照组比较,模型组大鼠血清铁调素、ET-1、ROS、MDA水平均升高(P<0.05、0.01),而NO、SOD及CAT水平均降低(P<0.05、0.01);主动脉内铁含量明显升高(P<0.01),且主动脉组织出现了内膜损伤。与模型组比较,肝素组和川芎嗪组大鼠血清铁调素、ET-1、ROS、MDA水平均显著降低(P<0.05、0.01),NO及CAT水平均显著升高(P<0.01),SOD水平有升高趋势;主动脉组织内铁含量均降低(P<0.05、0.01),且主动脉组织内膜损伤有所减轻。结论内皮损伤程度加重的同时铁调素呈高表达状态,应用川芎嗪后,内皮损伤减轻,铁调素水平下降,说明川芎嗪对内皮的保护作用可能与其对铁调素高表达的抑制有关。
Objective To investigate the correlation between vascular endothelial injury and high expression of hepcidin, and the effect of ligustrazine on it. Methods Twenty-four SD rats were randomly divided into 4 groups: control group, model group, heparin-positive control group (heparin group) and ligustrazine group, 6 rats in each group. Control rats were fed with normal feed, and the remaining three groups were fed with high fat diet. After 8 weeks, the rats in the control group and model group were treated with saline ip (2mL / kg ip), heparin sodium heparin sodium injection 5mg / kg (ip) and ligustrazine hydrochloride ip 40mg / kg . Serum samples were collected from the abdominal aorta of rats and the serum levels of serum lipids were measured. Serum hepcidin, nitric oxide (NO), endothelin-1 (ET-1), reactive oxygen species (MDA), catalase (CAT) and superoxide dismutase (SOD) levels were measured. The aorta tissue was intercepted and iron content was measured and histopathological observation was performed. Results Compared with the control group, the levels of serum hepcidin, ET-1, ROS and MDA in model group were significantly increased (P <0.05, 0.01), while the levels of NO, SOD and CAT were decreased ; The content of iron in the aorta was significantly increased (P <0.01), and the intima of the aorta was damaged. Compared with model group, serum hepcidin, ET-1, ROS and MDA in heparin group and ligustrazine group were significantly decreased (P <0.05, 0.01), NO and CAT levels were significantly increased (P <0.01) , And the level of SOD increased. The content of iron in the aorta was decreased (P <0.05, 0.01), and the aortic intima lesion lessened. Conclusions Endothelial injury is aggravated while hepcidin is highly expressed. After ligustrazine administration, the injury of endothelial cells and the decrease of hepcidin level may be related to the protective effect of Ligustrazine on the endothelium may be related to its inhibitory effect on the high expression of hepcidin.