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【摘 要】
：
目的 构建并验证一种可用作生物免疫治疗载体的非复制尿嘧啶营养缺陷型弓形虫(NRTUA),并鉴定其表型.方法 通过经典的基于同源重组的基因敲除技术构建稳定的NRTUA虫株;构建p-OM1-HXGPRT-OM2、p-OM1-OM2和p-UP1-HXGPRT-UP2质粒用于敲除RHΔku80Δhxgprt弓形虫中的乳清苷-5\\\'-磷酸脱羧酶(OMPDC)基因和尿苷磷酸化酶(UP)基因,并通过电转染的方法转染到RHΔku80Δ hxgprt弓形虫中,最后经过药物筛选和极限稀释得到RHΔku80ΔompdcΔ
【机 构】
：
中山大学中山医学院,广东广州510080;中山大学附属第一医院,广东广州510080;中山大学中山医学院,广东广州510080;中山大学热带病防治研究教育部重点实验室,广东广州510080
【出 处】
：
热带医学杂志
【发表日期】
：
2021年12期
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