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目的寻找生川乌的毒性基因,探讨其毒性机制。方法根据国际ICH的要求,在SPF实验条件下采用生川乌水煎液ig昆明种小鼠进行毒性实验。采用基因表达谱技术,就生川乌对小鼠5种脏器的毒性进行全基因组描绘,应用Cluster、GO和Pathway等生物信息学手段对获取的数据进行综合分析并进行定量PCR验证。结果生川乌明显影响小鼠黏着斑(Focal adhesion)通路中的黏附素(ECM)、局部黏附激酶(FAK)和GTP结合蛋白(Cdc42)等关键基因。结论生川乌可能是通过影响小鼠Focal adhesion信号通路的关键基因而引起毒性,最终导致毒性的产生。
Objective To search for virulence genes of Chuanwuwu and explore its toxicity mechanism. Methods According to the requirements of international ICH, the toxicity experiments were carried out on Kunming mice using iguanjian decoction ig under the SPF experimental conditions. Gene expression profiling was used to characterize the toxicity of Shengchuanwu to five kinds of organs in mice. Bioinformatics tools such as Cluster, GO and Pathway were used to analyze the data and carry out quantitative PCR. Results Shengchuanwu obviously affected the key genes such as adhesion kinase (ECM), local adhesion kinase (FAK) and GTP binding protein (Cdc42) in the Focal adhesion pathway in mice. Conclusions Shengchuanwu may cause toxicity through the key genes that affect the Focal adhesion signaling pathway in mice, and finally lead to the generation of toxicity.