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将新鲜山蘑菇的子实体7.3kg加入85％乙醇6L,混匀后放置过夜,提取液于40～45℃加热移去乙醇,得到的含水相中加入1L氯仿提取,分出氯仿层,于40～45℃加热得到干燥的固体4.99g。将此固体经柱层分离。取11～1 5流份,再经柱层分离,以苯-乙酸乙酯(6:4)洗脱,得3个流份,取第三个流份经柱层分离,得到4个流份,其中取第二个流份以氯仿-乙醚重结晶即得十八烯酸衍生物16.7mg。本品为白色结晶,mp118～50℃,分子量
The fruit body of fresh mountain mushroom 7.3kg was added to 65% ethanol 6L, mixed and placed overnight, the extract was heated at 40-45°C to remove the ethanol, the obtained aqueous phase was added 1L chloroform extraction, and the chloroform layer was separated at 40°C. Heating at -45°C gave 4.99 g of a dry solid. The solid was separated by column layer. Take 11 to 15 flow fractions, and then separated by column, eluted with benzene-ethyl acetate (6:4) to obtain 3 fractions. The third fraction was separated by column layer to obtain 4 fractions. , which takes the second fraction with chloroform-ether recrystallization to obtain octadecenoic acid derivative 16.7mg. This product is white crystal, mp118～50°C, molecular weight