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选择雄性 Wistar 大鼠,体重200-220g。经 Zn(1mmol/kg)或 Cd(9μmol/kg)预处理12h 后,处死动物。采用胶原酶法分离大鼠肝细胞,并立即经台盼蓝排斥实验,要求细胞悬液生存率大于85%,测定细胞内金属硫蛋白
Male Wistar rats were selected and weighed 200-220 g. After pretreatment with Zn (1mmol / kg) or Cd (9μmol / kg) for 12h, animals were sacrificed. Isolation of rat hepatocytes by collagenase method and immediately by trypan blue exclusion experiments required the survival rate of cell suspension greater than 85%, determination of intracellular metallothionein