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目的：建立群体反应性抗体（ＰＲＡ）的检测方法，分析术前ＰＲＡ检测在预测术后早期发生急性排斥反应中的价值；方法：以６２名健康成人外周血淋巴细胞作为群体淋巴细胞来源，依据补体依赖微量淋巴毒方法建立ＰＲＡ的检测方法。研究对象为１９９４年６月至１９９７年２月在我院行肾移植术的１２０例患者。结果：对１９９４年６月至１９９６年７月９８例患者的回顾性研究结果表明，随着ＰＲＡ水平的升高，术后一月内急性排斥的危险性也增高，７２％发生急性排斥反应的患者术前ＰＲＡ水平高于２０％；对１９９６年７月后患者的前瞻性研究结果表明，２２例患者中术前４例ＰＲＡ高于３０％，其中有３例术后一月内发生了急性排斥反应。结论：本实验室条件下建立的ＰＲＡ检测方法具有一定的稳定性和可靠性，对肾移植患者术后发生早期排斥反应有一定的预测价值。
OBJECTIVE: To establish a method for the detection of PRA, and to analyze the value of preoperative PRA in predicting acute rejection in early postoperative period.Methods: Totally 62 healthy adult peripheral blood lymphocytes were used as the source of population lymphocytes, Complement dependent detection of PRA by means of microlymph lymphotoxin method. The study was performed on 120 patients undergoing renal transplantation in our hospital from June 1994 to February 1997. Results: The retrospective study of 98 patients from June 1994 to July 1996 showed that with the increase of PRA level, the risk of acute rejection within one month after operation was also increased, and the incidence of acute rejection in 72% Preoperative PRA levels were above 20%; prospective studies of patients after July 1996 showed that among the 22 patients, PRA was higher than 30% in 4 of the 4 preoperative patients, 3 of whom were acute within 1 month of surgery Rejection. Conclusion: The PRA detection method established in our laboratory has some stability and reliability, and it has a certain predictive value in the early rejection after renal transplantation.