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目的研究大鼠尾加压素-II(RUII)收缩大鼠离体肺动脉干环与细胞信号转导通路蛋白激酶C通道的关系。方法从雄性Sprague-Dauley大鼠中分离出肺动脉干,切成3~4mm的血管环,用RUII(10nmol/L)预收缩血管达坪台期后,加入蛋白激酶C通道阻断剂H-7,制备H-7(0.1~100μmol/L)浓度—效应舒张曲线,最后分别计算EC50和Emax。结果H-7呈浓度依赖性舒张大鼠RUII预收缩的肺动脉干-log[EC50]=5.26±0.36,Emax=(97.21±17.86)%。结论细胞信号转导通路蛋白激酶C通道的激活参与大鼠尾加压素-II收缩大鼠肺动脉干效应。
Objective To investigate the relationship between the isolated pulmonary artery rings and the signal transduction pathway of protein kinase C in rats after urotensin-II (RUII) contraction. METHODS Pulmonary arterial stem cells were isolated from male Sprague-Dauley rats and cut into 3 ~ 4 mm vascular rings. After pre-contracting the vessel with RUII (10 nmol / L) to reach the plateau, protein kinase C channel blockers H-7 , The concentration-effect relaxation curve of H-7 (0.1-100 μmol / L) was prepared, and the EC50 and Emax were calculated respectively. Results Pulmonary arterial-log [EC50] was 5.26 ± 0.36 and Emax was (97.21 ± 17.86)% in HII-preconditioned rats with concentration-dependent relaxation. Conclusion Activation of protein kinase C channel in cell signal transduction pathway is involved in the pulmonary arterial function in rats with urotensin-II contraction.