论文部分内容阅读
目的探讨重型乙型肝炎患者感染的HBV基因型分布及各型HBVDNA CP区变异情况。方法应用乙肝病毒型特异性引物采用巢式PCR方法,对42例重型乙型肝炎患者进行乙型肝炎病毒基因型分析;用PCR方法和DNA测序方法检测CP区突变。结果42例重型乙型肝炎患者中,B基因型12例(28.57%),C基因型29例(69.05%),B+C型1例(2.38%),未发现其他基因型别;42例患者所感染HBV基因组发生前C区1896点突变有32例(76.19%),其中,C基因型有28例(87.5%),B基因型有4例(12.5%)。B型BCP区ntA1762T/ntG1764A双突变3例(25%),C型BCP区双突变26例(89.66%),B+C型(1例)未发生突变结论重型乙型肝炎患者以感染B、C基因型为主,C基因型较B基因型更易发生基因变异。
Objective To investigate the distribution of HBV genotypes and the variation of CP region of various types of HBV in patients with severe hepatitis B infection. Methods Hepatitis B virus genotypes were detected in 42 patients with severe hepatitis B by nested PCR using HBV-specific primers. The mutations in the CP region were detected by PCR and DNA sequencing. Results Among the 42 patients with severe hepatitis B, 12 (28.57%) were B genotypes, 29 (69.05%) were C genotypes and 1 (2.38%) were B + C genotypes. No other genotypes were found in 42 patients There were 32 (76.19%) of 1896 point mutations in the C region before the HBV genome was infected, of which 28 (87.5%) were C genotypes and 4 (12.5%) were genotype Bs. There were 3 cases (25%) of double mutations of ntA1762T / ntG1764A in B type B region, 26 cases (89.66%) of double mutations in type C BCP region and no mutation in B + C type (1 case). Conclusion The patients with severe hepatitis B were infected with B, C genotype, C genotype is more prone to gene mutation than B genotype.