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目的:建立披针叶黄华不同部位中金雀花碱及其衍生物的高效液相色谱分析方法。方法:采用Lichrospher Si-120A色谱柱(250 mm×4.6 mm,5μm),以甲醇-乙醚-5%氨水(46∶52∶2)为流动相,流速1.0 mL·min-1,检测波长310 nm,柱温为室温。结果:金雀花碱与其2个衍生物完全分离,金雀花碱、N-甲基金雀花碱和N-甲酰基金雀花碱在各自的线性范围内线性关系均良好(r≥0.9999),最低检测限分别为0.5、0.1、0.3μg·mL-1,分别测定了披针叶黄华不同部位中3个成分的含量。结论:该方法可用于披针叶黄华中金雀花碱及其衍生物的测定,为金雀花碱生产原料的采收提供了依据。
OBJECTIVE: To establish a HPLC method for the determination of ginsenoside and its derivatives in different parts of Phlox glabrata. METHODS: The mobile phase consisted of Lichrospher Si-120A column (250 mm × 4.6 mm, 5 μm) and methanol-diethyl ether-5% ammonia solution (46:52:2) at a flow rate of 1.0 mL · min- The column temperature was room temperature. Results: There was a complete separation of cytisine from its two derivatives. There was a good linear relationship between cytisine, N-methylcholine and N-formylcholine in their respective linear ranges (r≥0.9999) , The lowest detection limits were 0.5,0.1,0.3μg · mL-1, were measured in different parts of Phlox lanceolate three components in the content. Conclusion: This method can be used for the determination of ginsenoside and its derivatives in Phlox glabra plumbagin, which provides the basis for the recovery of raw materials of gonadal.