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基因转录调节是植物对非生物胁迫适应机制的一个重要方面,转录调节因子在胁迫信号转导途径中调节下游基因的表达,在建立植物对胁迫适应性过程中起到重要作用。锌指蛋白是功能多样的转录调节因子蛋白家族,家族成员在植物响应非生物胁迫方面扮演着重要角色。本研究以秋茄C2H2型锌指蛋白编码基因KcZFP为目的基因,在烟草中过表达KcZFP,分析C2H2型锌指蛋白在植物耐盐性中的作用。研究结果显示:转基因株系中,KcZFP表达量显著提高。过表达KcZFP的烟草植株的耐盐性明显提高,在200mmol/LNaCl处理的条件下,KcZFP过表达烟草中脯氨酸水平远高于野生型植株。对光合作用参数比较分析显示,在KcZFP过表达植株中净光合速率受盐胁迫的影响小于野生型植株,光合系统在一定程度上得到了保护。研究结果说明KcZFP作为转录调节因子参与了植物的渗透调节,对植物的耐盐性具有贡献。
Transcriptional regulation is an important aspect of adaptation mechanism of plants to abiotic stresses. Transcription regulators regulate the expression of downstream genes in stress signal transduction pathways and plays an important role in establishing plant adaptability to stress. Zinc finger proteins are a versatile family of transcriptional regulator proteins that play important roles in the plant response to abiotic stresses. In this study, KCZFP, a type of zinc finger protein of candel type C2H2, was overexpressed in tobacco and the role of C2H2 zinc finger protein in salt tolerance of plants was analyzed. The results showed that: KcZFP expression in transgenic lines significantly increased. The salt tolerance of tobacco plants overexpressing KcZFP increased significantly. Under 200 mmol / L NaCl treatment, the proline level in KcZFP overexpressing tobacco was much higher than that in wild type plants. Comparative analysis of photosynthetic parameters showed that the net photosynthetic rate in KcZFP overexpression plants was less affected by salt stress than the wild type plants, and the photosynthetic system was protected to a certain extent. The results showed that KcZFP participated in the osmotic adjustment of plants as a transcriptional regulator and contributed to the salt tolerance of plants.