论文部分内容阅读
通过测定原代培养的谷氨酸诱导的受损海马神经的细胞存活率,谷胱甘肽过氧化物酶活性,以及谷胱甘肽和一氧化氮的水平,研究多不饱和脂肪酸,如二十二碳六烯酸(docosahexaenoic acid,DHA)、花生四烯酸(arachidonic acid,AA),对大鼠海马神经细胞损伤的影响。海马神经元用神经特异性烯醇化酶进行鉴定。研究发现:(1)DHA 5～50 mg/L能有效的对抗谷氨酸诱导的海马神经细胞的损害作用。表现在细胞存活率、谷胱甘肽过氧化物酶的活性较谷氨酸处理组明显提高,NO水平降低,谷胱甘肽含量与对照组相比变化不大。(2)5 mg/L AA对损伤没有影响,而 30和 50 mg/L AA对损伤具有加重作用,其细胞存活率、GSH- Px活性、GSH水平较谷氨酸处理组明显降低。结果表明,多不饱和脂肪酸对谷氨酸诱导的神经细胞损伤具有双重作用。
Polyunsaturated fatty acids, such as bis (2-ethylhexyl) -1 -cyclodextrin, polyunsaturated fatty acids, glutathione peroxidase, Effects of docosahexaenoic acid (DHA) and arachidonic acid (AA) on neuronal damage in the hippocampus of rats. Hippocampal neurons were identified with neuro-specific enolase. The study found: (1) DHA 5 ~ 50 mg / L can effectively antagonize glutamate-induced damage of hippocampal neurons. The results showed that the cell viability, the activity of glutathione peroxidase was significantly higher than glutamate-treated group, the NO level was reduced, and the content of glutathione changed little compared with the control group. (2) 5 mg / L AA had no effect on the injury, while 30 and 50 mg / L AA had an aggravating effect on the injury. The cell viability, GSH-Px activity and GSH level were significantly lower than those of glutamate-treated group. The results show that polyunsaturated fatty acids have a dual effect on glutamate-induced neuronal damage.