三氧化二砷致突变实验研究

• 作 者: 肖传方 王喜正 唐素琴 余戴礼 王英德
• 机 构: 北京医科大学,北京医科大学,北京医科大学,广东医药学院,广东医药学院
• 出 处: 环境与健康杂志
• 发表日期: 1987年05期
• 关键词: 致突变物 阈剂量 小鼠骨髓细胞 染色体畸变率 昆明种小白鼠 空白对照组 微核率 大鼠 无机砷 环磷酞胺

本实验以As_2O_3为代表,研究了无机砷致突变的剂量—反应关系。进行了砷对小鼠骨髓细胞染色体畸变,骨髓多染红细胞微核率实验,及砷对人外周血淋巴细胞SCE频率和UDS效应实验。结果表明:5mg/kg(100mg/L)不引起UDS效应的显著改变;0.04～0.4mg/L虽可引起SCE频率增高,但均未超过对照组的2倍;0.5mg/kg以上可引起小鼠骨髓细胞染色体畸变率和骨髓多染红细胞微核率显著升高;As_2O_3致突变的阈剂量为0.5mg/kg。

In this experiment, As 2 O 3 was used as a representative to study the dose-response relationship of mutagenicity of inorganic arsenic. Arsenic on mouse bone marrow cell chromosome aberration, bone marrow erythrocytes micronucleus rate experiments, and arsenic on human peripheral blood lymphocytes SCE frequency and UDS effect experiments. The results showed that: 5mg / kg (100mg / L) did not cause a significant change in UDS effect; 0.04 ~ 0.4mg / L although can cause SCE frequency increased, but did not exceed 2 times the control group; 0.5mg / kg or more can cause small Chromosome aberration rate of bone marrow cells and micronuclei of erythrocytes in bone marrow were significantly increased. The threshold dose of mutagenicity of As_2O_3 was 0.5 mg / kg.