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为了研究咖啡酸锗对小鼠U14瘤的抑制作用及其细胞凋亡机制,试验采用MG-P染色、透射电镜和免疫组化方法观察了U14瘤细胞的凋亡及瘤细胞中Bax和Bcl-2蛋白表达情况。结果表明:MG-P染色及透射电镜观察可见,咖啡酸锗试验组出现较多的凋亡细胞,可见典型的细胞凋亡形态特征;免疫组化试验显示,咖啡酸锗各剂量组能下调Bcl-2蛋白表达(P<0.05),上调Bax蛋白表达(P<0.01或P<0.05)。说明咖啡酸锗能抑制小鼠U14瘤的生长,并能诱导U14瘤细胞凋亡,这可能是其发挥抗肿瘤作用的主要机制之一。
In order to study the inhibitory effect of caffeic acid germanium on mouse U14 tumor and its mechanism of apoptosis, we observed the apoptosis of U14 tumor cells and the expression of Bax and Bcl-2 in tumor cells by using MG-P staining, transmission electron microscopy and immunohistochemistry. 2 protein expression. The results showed that MG-P staining and transmission electron microscopy showed that most of the apoptotic cells appeared in the Caffeic acid-germanium test group, and typical morphological features of apoptosis were observed. Immunohistochemistry showed that Ca-Ca-Ge dose could reduce Bcl- -2 protein (P <0.05), and up-regulated Bax protein expression (P <0.01 or P <0.05). Caffeic acid germanium can inhibit the growth of U14 tumor cells and induce apoptosis of U14 tumor cells, which may be its anti-tumor effect of one of the main mechanisms.