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我们于1972年分离获得马尾松毛虫质型多角体病毒(简称 D.CPV)后,经室内毒力测定和林间防治试验,迄今已在揭西等10余个县(市)4万余亩面积对马尾松毛虫进行了推广性防治试验,获得了较好的效果。现将部份林间试验结果总结如下。一、材料与方法1.材料供试病毒为广东省林科所于1972年分离获得的 D.CPV16号。经过虫体复制,将虫尸加入50%甘油后保存于冰箱(-10℃),或把虫尸加工成粗提病毒悬液,加入50%甘油后保存于冰箱(2—4℃)1个
We isolated the Masson Dendrolimus punctatus polyhedrosis virus (referred to as D.CPV) in 1972, after indoor toxicity determination and forest prevention and control test, so far in more than 10 counties (cities) such as Jiexi more than 4 acres Area of Dendrolimus punctatus for the promotion of prevention and control tests, obtained good results. Now part of the forest test results are summarized below. First, the material and methods 1. Materials for the test virus for the Guangdong Provincial Forestry Branch in 1972, obtained D.CPV16. After the insect body copy, the dead body by adding 50% glycerol stored in the refrigerator (-10 ℃), or the worm processed into crude extract virus suspension, add 50% glycerol and kept in the refrigerator (2-4 ℃) 1