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目的:探讨血清及尿液生物标志物骨桥蛋白(Osteopontin,OPN)在膀胱尿路上皮癌诊断中的价值。方法:收集膀胱尿路上皮癌患者(n=40,研究组)、泌尿系良性疾病患者(n=20,良性组)及健康志愿者(n=10,对照组)血清和尿液标本,通过酶联免疫吸附试验(ELISA)检测OPN的表达量,分析OPN与临床病理特征的关系,并绘制ROC曲线确定诊断价值。结果:研究组血清及尿液OPN表达量均高于良性组和对照组(均P<0.05);膀胱尿路上皮癌患者血清OPN表达量与TNM分期、病理分级、是否转移相关(均P<0.05),而尿液OPN表达量与TNM分期、病理分级、是否转移无相关(均P>0.05);血清、尿液OPN对膀胱尿路上皮癌诊断的敏感性分别为90.2%、78.0%,特异性分别为84%、84%。结论:OPN在膀胱尿路上皮癌中具有一定的潜在诊断价值,在患者血清及尿液中显著增高,与临床病理特征密切相关。
Objective: To investigate the value of serum and urine biomarker osteopontin (OPN) in the diagnosis of bladder urothelial carcinoma. Methods: Serum and urine samples were collected from patients with bladder urothelial carcinoma (n = 40, study group), patients with benign urologic disease (n = 20, benign group) and healthy volunteers (n = 10, The expression of OPN was detected by enzyme linked immunosorbent assay (ELISA), the relationship between OPN and clinicopathological features was analyzed, and the ROC curve was drawn to confirm the diagnostic value. Results: The expression of OPN in serum and urine of study group were higher than those in benign group and control group (all P <0.05). The expression of OPN in bladder urothelial carcinoma was correlated with TNM stage, pathological grade and metastasis (all P < 0.05). The OPN expression in urine was not related to TNM staging, pathological grade and metastasis (all P> 0.05). The sensitivity of serum and urine OPN in diagnosing bladder urothelial carcinoma were 90.2% and 78.0% Specificity was 84%, 84%. Conclusion: OPN has some potential diagnostic value in bladder urothelial carcinoma, which is significantly increased in serum and urine of patients, which is closely related to clinicopathological features.