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目的研究油茶皂素7、8对人胃癌SGC-7901细胞的增殖抑制作用及凋亡观察。方法常规培养SGC-7901细胞,采用噻唑蓝(MTT)法检测细胞的活性,而后选用Hoechst33342染色观察细胞的凋亡形态,最后选用AnnexinV/PI双标记法检测细胞的凋亡。结果 5～100μg·mL-1油茶皂素7、20～200μg·mL-1油茶皂素8对人胃癌SGC-7901细胞的抑制作用表现出明显的剂量依赖关系;50μg·mL-1油茶皂素7、100μg·mL-1油茶皂素8处理过的细胞被染色后,部分细胞核内呈现浓染致密的颗粒块状荧光或荧光碎片,表明SGC-7901细胞发生了凋亡;50μg·mL-1油茶皂素7可显著诱导SGC-7901的早期凋亡,早期凋亡率为4.33%,总凋亡率(8.1%)明显大于空白对照组(4.89%),150μg·mL-1油茶皂素8可明显诱导SGC-7901细胞的早期凋亡(2.46%)和晚期凋亡(5.69%)。结论油茶皂素7、8可剂量依赖性抑制SGC-7901细胞的增殖活性,并能诱导SGC-7901细胞凋亡。
Objective To study the inhibitory effect of Camellia saponin 7,8 on the proliferation of human gastric cancer cell line SGC-7901 and its apoptosis. Methods SGC-7901 cells were cultured routinely. The cell viability was measured by MTT assay. Then the apoptosis morphology was observed by Hoechst33342 staining. Finally, the apoptosis of SGC-7901 cells was detected by Annexin V / PI double labeling method. Results The inhibitory effect of 5-100μg · mL-1 Camellia saponin 7,20 ~ 200μg · mL-1 Camellia saponin 8 on human gastric cancer SGC-7901 cells showed a significant dose-dependent relationship; 50μg · mL-1 Camellia saponin 7,100μg · mL-1 camellia saponin 8-treated cells were stained, some nuclei showed dense dense granular lumps of fluorescent or fluorescent debris, indicating apoptosis of SGC-7901 cells; 50μg · mL-1 The early apoptosis rate of SGC-7901 was 4.33% and the total apoptosis rate (8.1%) was significantly higher than that of the blank control group (4.89%), 150μg · mL-1 camellia saponin 8 SGC-7901 cells could significantly induce early apoptosis (2.46%) and late apoptosis (5.69%). Conclusion Camellia saponin 7,8 can inhibit the proliferation of SGC-7901 cells in a dose-dependent manner and induce the apoptosis of SGC-7901 cells.