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籼型杂交稻组合“汕优23选”为新的具高培养力的材料,其幼穗愈伤组织诱导率约为80%,绿苗得率高达970.8%。幼穗离体培养选材以穗长2cm 以下(幼穗分化第3～4期)为宜。MN 和 N_6两种培养基适用于籼型杂交稻的幼穗离体培养,以 MN(诱导)→N_6(继代)→MN(分化)的培养基替换方式,对愈伤组织的继代培养和绿苗分化具有良好效果。采用KT 2mg/L+BA 1mg/L+IAA 0.5mg/L+NAA 0.5mg/L 的激素配比,有效地提高了绿苗分化率。通过幼穗离体培养,可迅速和大量地获得再生植株,这对开展水稻组培育种和体细胞遗传学研究具有一定意义。
The indica hybrid rice combination “Shanyou 23” was a new material with high culture ability. The young calli induction rate was about 80% and the green seedling rate was 970.8%. Spikelet in vitro culture selection to spike length 2cm below (panicle differentiation of the first 3 to 4) is appropriate. MN and N_6 were suitable for young in vitro culture of Indica hybrid rice. The subculture of callus was carried out with the medium of MN (induced) → N_6 (subculture) → MN (differentiation) And green shoot differentiation has good effect. The hormone ratio of KT 2mg / L + BA 1mg / L + IAA 0.5mg / L + NAA 0.5mg / L effectively increased the rate of green plantlet differentiation. Through in vitro culture of young spikelets, regenerated plants can be rapidly and abundantly obtained, which is of great significance for the study of tissue culture and somatic cell genetics in rice.