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目的:确定尿沉淀细胞DNA中的13个肿瘤相关基因启动子的甲基化谱式分析在膀胱癌诊断中的价值。方法:用定性甲基化特异性(methylation specific polymerase chain reaction,MSP)的方法,对92例临床确诊的膀胱癌患者、23例非肿瘤性尿路疾病患者、6例脑外科患者、7例健康志愿者检测了尿沉淀细胞DNA中肿瘤相关基因启动子的甲基化状态。结果:在临床确诊的92例膀胱癌患者中被检测的13个基因的高甲基化状态出现频率显著高于23例非肿瘤性尿路疾病患者,差异有统计学意义(P<0.05)。而6例脑外科患者和7例正常健康人的尿沉淀细胞DNA中,上述基因均为去甲基化状态。若以任一个基因高甲基化为膀胱癌的指征,88.0%(81/92例)的膀胱癌可被检出。结论:MSP法分析尿沉淀细胞DNA中肿瘤相关基因启动子的甲基化状态可有效地检出膀胱癌。
OBJECTIVE: To determine the methylation profile of 13 tumor-associated gene promoters in urinary sediment cell DNA in the diagnosis of bladder cancer. Methods: A total of 92 clinically diagnosed bladder cancer patients, 23 non-neoplastic urological disease patients, 6 cerebral surgery patients, and 7 healthy controls were studied by methylation-specific polymerase chain reaction (MSP) Volunteers examined the methylation status of the tumor-associated gene promoter in urine-precipitated cell DNA. Results: The frequency of hypermethylation of the 13 genes detected in 92 bladder cancer patients with clinical diagnosis was significantly higher than that in 23 patients with non-neoplastic urinary disease (P <0.05). In the urine sedimentation cell DNA of 6 brain surgery patients and 7 normal healthy people, the above genes were all demethylated. 88.0% (81/92) of bladder cancers could be detected if any of the genes were hypermethylated to bladder cancer. Conclusion: MSP method can detect urinary sediment cell DNA methylation status of tumor-related gene promoter can effectively detect bladder cancer.