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目的：探索提高胶质瘤治疗效果的新方法。方法：以化学偶联制备抗ＣＤ３－抗胶质瘤双特异性抗体，以３Ｈ掺入法测定其与ＩＬ－２协同增强ＬＡＫ细胞对胶质瘤的细胞毒性，以ＣＤ３单抗、胶质瘤单抗、二种单抗混和物及ＲＰＭＩ１６４０作对照。结果：双特异抗体与单抗相比，能显著提高ＬＡＫ细胞对胶质瘤细胞的杀伤作用；双抗加入ＩＬ－２后，ＬＡＫ细胞毒性得到显著的提高，ＩＬ－２也能增强抗ＣＤ３单抗和ＳＺ３９单抗的细胞毒性；同时发现，来源于恶性胶质瘤患者的ＬＡＫ细胞毒性，在加入单抗、双抗和ＩＬ－２后，其细胞毒性与正常人相比，仍有显著性差异。结论：抗ＣＤ３－抗胶质瘤双特异抗体与ＩＬ－２协同可显著提高ＬＡＫ细胞对胶质瘤的细胞毒作用
Objective: To explore new ways to improve the therapeutic effect of glioma. METHODS: The anti-CD3-anti-glioma bispecific antibody was prepared by chemical coupling and its cytotoxicity to IL-2 was enhanced by 3H incorporation. The cytotoxicity of LAK cells against glioma was assessed by CD3 monoclonal antibody and glioma. Monoclonal antibodies, two monoclonal antibody mixtures and RPMI1640 were used as controls. RESULTS: Compared with the monoclonal antibody, bispecific antibody can significantly increase the killing effect of LAK cells on glioma cells; LAK cytotoxicity was significantly increased after the anti-IL-2 antibody was added to the anti-CD3 monoclonal antibody. Anti- and SZ39 monoclonal antibody cytotoxicity; also found that LAK cytotoxicity from malignant glioma patients, after the addition of monoclonal antibody, double antibody and IL-2, its cytotoxicity compared with normal people, there is still significant difference. Conclusion: Anti-CD3-anti-glioma bispecific antibody and IL-2 can significantly increase the cytotoxicity of LAK cells to glioma