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目的探讨氯沙坦对系膜细胞增殖的影响以及对血小板源生长因子(PDGF)所诱导的细胞增殖作用。方法取传代培养的大鼠肾小球系膜细胞,分别加入不同浓度的PDGF、血管紧张素Ⅱ(AngⅡ)和氯沙坦,用BrdU掺入法和四甲基偶氮唑盐(MTT)法测定细胞增殖的情况。结果PDGF和AngⅡ明显促进系膜细胞的生长(P<0.01);氯沙坦(10-7~10-5mol/L)有抑制系膜细胞增殖的作用(P<0.01),与维拉帕米联合使用则抑制作用更为明显;PDGF组加入氯沙坦后,系膜细胞增殖明显减弱,与单纯PDGF组比较,有显著差异(P<0.01)。结论PDGF具有明确的促进大鼠肾小球系膜细胞增殖作用。氯沙坦则抑制系膜细胞增殖活性,可能是通过阻断PDGF的诱导增殖作用而介导的。
Objective To investigate the effects of losartan on the proliferation of mesangial cells and the proliferation of cells induced by platelet derived growth factor (PDGF). Methods Rat mesangial cells were subcultured with different concentrations of PDGF, Ang Ⅱ and losartan, respectively. BrdU incorporation and MTT Cell proliferation was measured. Results PDGF and Ang Ⅱ significantly promoted the proliferation of mesangial cells (P <0.01). Losartan (10-7 ~ 10-5 mol / L) inhibited the proliferation of mesangial cells (P <0.01) Combined use of the inhibition was more obvious; PDGF group added losartan, mesangial cell proliferation was significantly reduced, compared with the PDGF group, there was a significant difference (P <0.01). Conclusion PDGF has a clear promotion of rat mesangial cell proliferation. Losartan inhibited mesangial cell proliferation activity, which may be mediated by blocking the induction of PDGF proliferation.