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目的探讨丙泊酚对内毒素(LPS)性大鼠急性肺损伤(ALI)的影响。方法 60只SD大鼠随机均分5组:A组为ALI模型;B1、B2、B3组静脉注射LPS5mg/kg后,再分别输注丙泊酚5、10、15mg.kg-1.h-1;C组为假手术对照。放血处死大鼠,右肺HE染色,左肺测定湿干重比;分离外周血中性粒细胞(PMN),流式细胞仪检测PMN凋亡。结果与C组比较,A组肺部损伤严重,肺泡损伤比值(IQA)显著增加、PMN的凋亡率显著降低(P<0.05);与A组比较,丙泊酚处理组肺损伤程度减轻,IQA降低,凋亡显著增加,且呈剂量依赖性(P<0.05)。结论丙泊酚减轻LPS诱导的大鼠ALI,这可能与促进PMN的凋亡有关。
Objective To investigate the effect of propofol on acute lung injury (ALI) induced by endotoxin (LPS) in rats. Methods Sixty Sprague-Dawley rats were randomly divided into five groups: group A was model of ALI; group B, B and B were intravenously injected with 5 mg / kg LPS, and then received propofol 5, 10 and 15 mg.kg-1.h- 1; C group as sham control. The rats were sacrificed by exsanguination, the right lung was stained by HE and the wet / dry weight ratio was measured by left lung. Peripheral blood neutrophil (PMN) was isolated and the apoptosis of PMN was detected by flow cytometry. Results Compared with group C, group A had severe lung injury, significantly increased alveolar damage ratio (IQA), and apoptosis rate of PMN significantly decreased (P <0.05). Compared with group A, the degree of lung injury in propofol group was reduced, IQA decreased, apoptosis increased significantly, and in a dose-dependent manner (P <0.05). Conclusion Propofol attenuates LPS-induced ALI in rats, which may be related to the promotion of PMN apoptosis.