论文部分内容阅读
目的：观察我国1993～1994年试生产的肾综合征出血热（HFRS）沙鼠肾细胞Ⅰ型灭活疫苗（上海生研所和杭州天元公司生产）、乳鼠脑纯化Ⅰ型灭活疫苗（兰州生研所生产）、地鼠肾细胞Ⅱ型灭活疫苗（长春生研所生产）的安全性，考核血清学、近期和中期流行病学效果。方法：采用IFAT法检测特异性IgG抗体，用MCPENT法检测中和抗体滴度。结果：3种疫苗安全性皆较好，上海沙鼠苗、天元沙鼠苗、鼠脑苗和地鼠苗中强反应率分别为0.56％、0．03％、3.26％、1.57％。3种疫苗3针全程免疫后14天、加强前、加强后14天和1年，中和抗体阳转率平均为：地鼠苗78．36％、12.33％、80．56％和54．46％，上海沙鼠苗57．81％、10.79％、70．71％和35.58％，天元沙鼠苗70.00％、50．00％、91．18％和未到期，鼠脑苗51．09％、10.48％、61．90％和45.00％；荧光抗体阳转率地鼠苗为65．73％、33．90％、85．00％和49.44％，上海沙鼠苗93．69％、17．76％、89．04％和51.61％，天元沙鼠苗83．33％、12.90％、64．52％和未到期，鼠脑苗84．27％、12.50％、81.25％和0％。3种疫苗近期（基础免疫后1年）和中期（基础免疫后2年）流行病学防病效果均较好，其平均保护率：地鼠苗分别为97．81％、88．73％，上海沙鼠苗94.08％、92.17％，天元
Objective: To observe the type I inactivated vaccine of rat renal cell hemorrhagic fever with renal syndrome (HFRS) from 1993 to 1994 in China (Shanghai Institute of Health and Hangzhou Tianyuan Company), brain purified type I inactivated vaccine (Lanzhou Health Institute production), hamster kidney cell type II inactivated vaccine (Changchun Institute of Health) safety, evaluation of serology, recent and mid-term epidemiological effects. Methods: The specific IgG antibody was detected by IFAT method and the titer of neutralizing antibody was detected by MCPENT method. Results: The safety of the three vaccines was good. The strong response rate was 0.56%, 0.03%, 3.26% and 1.57% respectively in the gerbil, gerbil, tiger seedling and hamster seedlings. The three vaccines 3 days after the full immunization 14 days, before strengthening, strengthening 14 days and 1 year, the average rate of neutralization antibody positive rate: hamster seedlings 78.36%, 12.33%, 80.56% and 54.46 %, Shanghai gerbil seedlings 57.81%, 10.79%, 70.71% and 35.58%, 70.00%, 50.00%, 91.18% , 10.48%, 61.90% and 45.00%, respectively. The positive rate of fluorescent antibody was 65.73%, 33.90%, 85.00% and 49.44% respectively in hamster seedlings and 93.69% in Shanghai gerbil seedlings. 76%, 89.04% and 51.61% respectively. The gerbils of Tianyuan gerbils were 83.33%, 12.90%, 64.52% and unexpired, 84.27%, 12.50%, 81.25% and 0% respectively. The three kinds of vaccines have a good epidemiological and disease prevention effect in the short term (one year after basic immunization and two years after basic immunization), and the average protection rate of the three vaccines is 97.81% and 88.73% respectively. Shanghai gerbil seedlings 94.08%, 92.17%, Tianyuan