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以4个不同类型的水稻品种为材料,采用盆栽和营养液培养方法,研究了适度水分胁迫(5%PEG处理)和增硝营养对不同水稻品种幼苗氮素吸收积累、氮代谢关键酶及干重的影响。结果表明,在正常水分条件下,随营养液中NO3--N比例的增加,各水稻品种叶片及根部的NO3--N含量及NR活性均不同程度增加,AA-N含量、氮累积量以及GS、GOGAT和GDH活性则呈下降趋势,叶片中SP含量、GOT和GPT活性也呈下降趋势;在适度水分胁迫(5%PEG处理)下,适当增硝(铵硝配比50∶50)可促进叶片和根中GDH、GS、GOGAT、GPT活性(中旱3号除外)以及叶片中SP含量的提高,有利于增强对氨的同化能力。与正常水分处理相比,适度水分胁迫(5%PEG处理)能不同程度增加各品种叶和根中的NO3--N和AA-N含量及NR、GDH活性,叶中GS和GOGAT活性及根中GPT活性和根干重也呈增加趋势,而叶片和根中的GOT活性和氮累积量、根中GS和GOGAT活性以及叶中GPT活性整体上表现下降。不同水稻品种对水分胁迫和铵硝配比处理的响应存在差异,叶片和根部的NO3--N、SP含量和NR活性及叶中AA-N含量均以中旱3号最高,而叶片和根中GDH、GS和GOGAT活性、单株氮累积量和秧苗干重则以冈优527和扬稻6号较高。
Four different types of rice varieties were used as materials to study the effects of moderate water stress (5% PEG treatment) and increasing nitrate nutrition on nitrogen uptake and accumulation, nitrogen metabolism key enzymes and dry matter in different rice varieties Heavy influence. The results showed that NO3 - N content and NR activity in leaves and roots of rice varieties increased with the increase of NO3 - N ratio in nutrient solutions under normal water conditions. AA - N content, nitrogen accumulation, The activity of GS, GOGAT and GDH showed a downward trend, and the content of SP, GOT and GPT in leaves also showed a downward trend. Under suitable water stress (5% PEG treatment), the appropriate increase of nitrate (ammonium nitrate ratio 50:50) Promoting the activities of GDH, GS, GOGAT and GPT in leaves and roots (except Zhonghan 3) and increasing the content of SP in the leaves were beneficial to enhancing the assimilation ability of ammonia. Compared with normal water treatment, moderate water stress (5% PEG treatment) could increase NO3 - N and AA-N content, NR and GDH activity in leaves and roots, GS and GOGAT activity in leaves and roots GPT activity and root dry weight tended to increase, while GOT activity and nitrogen accumulation in leaves and roots, GS and GOGAT activity in roots and GPT activity in leaves decreased overall. The responses of different rice cultivars to water stress and ammonium nitrate were different. The content of NO3 - N and SP and the activities of NR and the contents of AA - N in leaves and roots were the highest in Zhonghan 3, The activities of GDH, GS and GOGAT, the accumulation of nitrogen per plant and the dry weight of seedlings were higher in Gangyou 527 and Yangdao 6.