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采用Ｐｕｌｓｉｎｅｌｌｉ４血管闭塞法（４ＶＯ）的改良法建立大鼠血管性痴呆（ＶＤ）动物模型，用Ｔ型水迷宫试验检查动物的学习和记忆能力，并以放射免疫测定法测定ＶＤ大鼠４个脑区（额叶皮质、海马、丘脑和纹状体）和周围血浆中β－内啡肽（β－ＥＰ）含量。结果表明，ＶＤ组大鼠出现严重的学习、记忆障碍的同时其额叶皮质、海马丘脑和纹状体中β－ＥＰ含量分别为相应对照组的４１％、６６％、６８％和４３％，比对照组显著降低（降低３２％～５９％，Ｐ＜０．０５）。血浆β－ＥＰ含量在两组间则无显著差异。提示ＶＤ动物学习与记忆障碍可能与脑缺血后多脑区内β－ＥＰ含量降低有关。
The animal model of vascular dementia (VD) was established by the modified method of Pulsinelli4 vessel occlusion (4VO). The learning and memory abilities of animals were examined by T-type water maze test. The four brains of VD rats were determined by radioimmunoassay (Prefrontal cortex, hippocampus, thalamus and striatum) and peripheral plasma beta-endorphin (beta-EP) content. The results showed that β-EP content in frontal cortex, hippocampal thalamus and striatum were 41%, 66%, 68% and 43% of the corresponding control group, while VD rats had severe learning and memory impairment. Significantly lower than the control group (reduced by 32% -59%, P <0.05). There was no significant difference in plasma β-EP levels between the two groups. Suggesting that VD animal learning and memory impairment may be related to the decrease of β-EP content in the multiple brain regions after cerebral ischemia.