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目的:分析烤瓷冠修复初期变形链球菌(Ua)、内氏放线菌(An)和牙龈卟啉单胞菌(Pg)在修复体表面黏附的先后顺序和定植数量,为临床上选择合适的修复体和尽可能减少细菌在修复体表面附着和定植提供参考。方法:将烤瓷材料制作完成并逐级打磨抛光后,和釉质一起放入分别含有上述3种细菌的混合培养基中厌氧培养,在6、12和24 h3个时间段,分别定量检测试件表面黏附的细菌数量以及液体培养基中的细菌数量,采用SPSS 6.0软件包对数据进行统计学处理。结果:2种试件表面及液体培养基中均未检测到Pg。前6 h,Ua/An在2组试件表面的附着烤瓷组少于釉质组;12 h后,An在烤瓷表面增加最快,釉质次之;24 h后,烤瓷组和釉质组的Ua/An几乎无变化。结论:3种细菌混合培养时,Pg受到强烈抑制。烤瓷修复初期,不需考虑对牙龈卟啉单胞菌的抑制。如要抑制Ua/An,对烤瓷修复体而言,前12 h是关键期。烤瓷冠是可优先选择的良好修复体。
OBJECTIVE: To analyze the sequence and number of colonization on the surface of prostheses after the restoration of porcelain crowns, and to choose the appropriate Restoration and minimizing the bacteria attached to the prosthesis surface and colonization provides a reference. Methods: The porcelain material was prepared and polished step by step. After enamel was placed in the mixed culture medium containing the above three kinds of bacteria respectively, anaerobic culture was carried out. After 6, 12 and 24 h, The number of bacteria adhering to the surface and the number of bacteria in the liquid medium were calculated using SPSS 6.0 software package. Results: Pg was not detected on the surface of both specimens and in liquid medium. In the first 6 h, Ua / An was less than the enamel group on the surface of the two specimens. After 12 h, An increased on the surface of the porcelain, followed by the enamel. After 24 h, the porcelain group and the enamel group Ua / An almost no change. Conclusion: Pg is strongly inhibited when mixed with three kinds of bacteria. In the early stage of porcelain restoration, there was no need to consider the inhibition of P. gingivalis. To suppress Ua / An, for the porcelain restorations, the first 12 h is the key period. Porcelain crown is a good choice for the restoration of body.