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为探究获得大量高纯度雪旺细胞的方法 ,取生后 1～ 3天SD乳鼠的坐骨神经 ,采用改进后的植块反复移植法进行细胞培养。同时通过低浓度胰酶快速消化法、差速贴壁法和加用细胞毒药物等去除成纤维细胞 ,纯化雪旺细胞。最后用抗S 10 0蛋白酶联免疫细胞化学染色法进行细胞鉴定。结果发现 ,经植块反复移植和多种方法结合纯化后 ,雪旺细胞的纯度可达 95 %以上 ,并具有良好的活性。故认为 ,此方法简便易行 ,可获得高纯度大量的乳鼠雪旺细胞
In order to explore a method for obtaining a large number of high-purity Schwann cells, the sciatic nerve of SD suckling rats 1 to 3 days after birth was cultured by the improved method of repeated graft transplantation. At the same time, fibroblasts were purified and purified by low-concentration trypsin digestion, differential adherent method and addition of cytotoxic drugs. Finally, anti-S 10 0 protein immunocytochemistry staining for cell identification. The results showed that the purity of Schwann cells can reach more than 95% after being repeatedly implanted by the grafts and purified by various methods, and has good activity. Therefore, this method is simple and easy to obtain a large number of high-purity neonatal rat Schwann cells