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利用茎尖外植体进行常绿杜鹃的试管繁殖亦已报道,但有关落叶杜鹃离体繁殖的报道甚少,故对此作了进一步的研究。本文阐述了落叶杜鹃花三个耐寒品系800374、620014、800057从茎尖外植体(芽梢)快速成芽的培养条件,即培养基成分、细胞分裂素及生长素水平、每周更换新鲜培养基及培养基的pH值等。 材料与方法 外植体的准备及培养物的定植 实验中所用的植物材料是生长在照光24小时(用白炽灯延长光照)的温室中的越南杜鹃(Rho—dodendron annamense Rehd.)三个耐寒品系800374、620014、800057。准备外植体时,先从植株上剪取芽梢2—3厘米长,除去包裹顶端分生组织的叶片,让芽梢浸泡在1克·升~(-1)的灭菌灵溶液(50％WP)中10分钟,再在0.5％次氯酸钠(10％市售漂白粉)中表面消毒15分钟(每升消毒液中加30滴吐
In vitro fertilization of cuckoo cucumbers has also been reported. However, few reports have been reported on the in vitro propagation of cuckoo cuckoo. Therefore, further studies have been carried out. This article describes the three cold-tolerant Rhododendron strains 800374,620014,800057 from the shoot tip explants (buds) rapid budding conditions, namely, the composition of the medium, cytokinin and auxin levels were changed weekly fresh culture Base and medium pH value. MATERIALS AND METHODS Preparation of explants and colonization of cultures The plant material used in the experiment was three cold-tolerant strains of Rho-dodendron annamense Rehd., Grown in a greenhouse light-shining for 24 hours (prolonged lighting with incandescent lamps) 800374,620014,800057. When preparing the explants, the buds are first cut 2-3 cm in length from the plant to remove the leaves of the apical meristem and the shoots are soaked in a solution of 1 g · L -1 sterilized spirit (50 % WP) for 10 minutes and then surface sterilized in 0.5% sodium hypochlorite (10% commercial bleach) for 15 minutes (30 drops per liter of disinfectant