论文部分内容阅读
目的研究肾小球系膜细胞的肿瘤坏死因子的自分泌功能，肿瘤坏死因子对肾小球系膜细胞作用的机制。方法利用体外培养的人和大鼠的肾小球系膜细胞，通过逆转录－多聚酶链反应、原位杂交和免疫组化方法，探讨它们之间的关系。结果肾小球系膜细胞既可产生肿瘤坏死因子，又有肿瘤坏死因子受体。结论肾小球系膜细胞是肿瘤坏死因子作用的靶细胞，肿瘤坏死因子通过旁分泌和自分泌两种途径作用于肾小球系膜细胞。
Objective To investigate the autocrine function of tumor necrosis factor in mesangial cells and the mechanism of tumor necrosis factor on mesangial cells. Methods The mesangial cells of human and rat cultured in vitro were used to investigate the relationship between them by reverse transcription-polymerase chain reaction, in situ hybridization and immunohistochemistry. Results Mesangial cells can produce both tumor necrosis factor and tumor necrosis factor receptors. Conclusion Mesangial cells are the target cells of tumor necrosis factor. Tumor necrosis factor acts on mesangial cells through paracrine and autocrine pathways.