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目的 8 异前列腺素F2α(8 iso PGF2α)是一种敏感、特异性反映缺血 再灌注后氧自由基增加的生 化指标。抗氧化剂N 乙酰半胱氨酸(NAC)具有清除氧自由基的作用。本研究旨在分析实验性心肌缺血大鼠血浆 与心肌8 iso PGF2α相关性以及NAC的治疗效果,探讨血浆8 iso PGF2α反映心肌氧自由基损伤程度的可能性和 NAC的干预效果。方法 45只雄性成年Wistar大鼠随机分为3组(每组15只):对照组、缺血组和NAC组。缺血 组和NAC组腹腔注射垂体后叶素(20U/kg)制成大鼠急性心肌缺血模型,以心电图上ST段的抬高作为心肌缺血 的指标。对照组仅腹腔注射生理盐水。NAC组缺血前2周开始用NAC(每日0.1g/kg)灌胃,共3周。应用ELISA 方法测定各组大鼠血浆及心肌组织8 iso PGF2α含量。结果 缺血组大鼠的血浆和心肌组织含量分别为(187.1± 45.8)pg/mL和(259.3±47.5)pg/g,明显高于正常对照组(60.4±13.7)pg/mL和(88.6±16.9)pg/g (P<0.01);NAC组的血浆和心肌组织8 iso PGF2α含量为(88.2±16.4)pg/mL和(109.4±24.7)pg/g明显低于 缺血组(P<0.01)。血浆与心肌8 iso PGF2α水平相关(r=0.856,P<0.01)。与正常组比较,缺血组的心电图 ST段明显抬高(心肌缺血45min时抬高最为明显,达0.34±0.05mV)(P<0.05);NAC组大鼠心
Objective 8 IsoPGF2α is a sensitive and specific biochemical indicator of oxygen free radical increase after ischemia-reperfusion. Antioxidant N acetylcysteine (NAC) has the effect of scavenging oxygen free radicals. The aim of this study was to investigate the association of plasma iso8GF2α and the therapeutic effect of NAC in rats with experimental myocardial ischemia and to explore the possibility of 8 iso PGF2α in plasma reflecting the extent of myocardial oxygen free radical damage and the intervention effect of NAC. Methods Forty-five male adult Wistar rats were randomly divided into three groups (n = 15): control group, ischemia group and NAC group. Rats in ischemia group and NAC group were injected with pituitrin (20U / kg) into the model of acute myocardial ischemia, and the elevation of ST segment on the electrocardiogram was used as an indicator of myocardial ischemia. The control group received intraperitoneal injection of saline only. NAC group 2 weeks before ischemia with NAC (0.1g / kg daily) for 3 weeks. ELISA method was used to determine the content of iso PGF2α in plasma and myocardial tissue in each group. Results Plasma and myocardial tissue contents in the ischemic group were (187.1 ± 45.8) pg / mL and (259.3 ± 47.5) pg / g, respectively, significantly higher than those in the normal control group (60.4 ± 13.7 pg / mL and 88.6 ± (P <0.01). The levels of iso PGF2α in plasma and myocardium in NAC group were significantly lower than those in ischemic group (88.2 ± 16.4 pg / mL and 109.4 ± 24.7 pg / g, P <0.01) ). Plasma and myocardial iso-PGF2α levels were correlated (r = 0.856, P <0.01). Compared with the normal group, ST segment in the ischemic group was significantly elevated (the most obvious elevation was 45min after myocardial ischemia, reaching 0.34 ± 0.05mV) (P <0.05); NAC group