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背景:脑血管疾病有多种发病机制,自由基及其脂质过氧化作用参与了脑缺血后神经细胞的损害过程。补阳还五汤是一种临床上常用的治疗缺血性脑血管病中药,其作用机制还有许多不明之处。目的:观察补阳还五汤及拆方对脑缺血再灌注模型大鼠血清及脑组织超氧化物歧化酶(SOD)、丙二醛及一氧化氮含量影响,探讨其作用机制,并研究方剂配伍的意义。设计:以实验动物为研究对象,随机对照的实验研究。单位:一所中医学院医院的神经内科。材料:实验在河南省中医研究院国家中医管理局三级实验室进行研究,选择上海西普尔-必凯实验动物有限公司提供的清洁级SD大鼠60只。干预:60只大鼠随机分为假手术组、模型组、活血组、黄芪组、总方组。通过四血管结扎法制作SD大鼠脑缺血再灌注模型,分别给予活血组药物8g/kg、黄芪组药物40g/kg、总方组药物48g/kg,假手术组、模型组给予生理盐水20mL/kg。取血清及脑组织匀浆应用黄嘌呤氧化酶法测定SOD,用硫代巴比妥酸法测定丙二醛。采用硝酸还原酶法测定一氧化氮的含量。主要观察指标:各组大鼠血清、脑组织超氧化物歧化酶(SOD)、丙二醛及一氧化氮含量。结果:模型组大鼠血清、脑组织丙二醛及一氧化氮含量明显增高(P<0.001～0.05),SOD含量明显降低(P<0.05,P<0.01);总方组与拆方黄芪组、活血组?
BACKGROUND: There are many pathogenesis of cerebrovascular diseases. Free radicals and lipid peroxidation are involved in the damage process of neurons after cerebral ischemia. Buyang Huanwu Decoction is a commonly used traditional Chinese medicine for the treatment of ischemic cerebrovascular disease, and its mechanism of action remains unclear. Objective: To observe the effect of Buyang Huanwu Decoction and Separating Prescription on the levels of superoxide dismutase (SOD), malondialdehyde, and nitric oxide in serum and brain tissue of rats with cerebral ischemia-reperfusion, and to explore its mechanism of action. The significance of prescription compatibility. Design: A randomized controlled experimental study of experimental animals. Unit: Department of Neurology, a Chinese Medical College Hospital. MATERIALS: Experiments were conducted in the third-level laboratory of the State Administration of Traditional Chinese Medicine of the Henan Academy of Traditional Chinese Medicine, and 60 clean grade SD rats were provided by Shanghai Xipu-Bikai Experimental Animal Co., Ltd. Intervention: 60 rats were randomly divided into sham operation group, model group, Huoxue group, Huangqi group, and Fang group. The model of cerebral ischemia-reperfusion in SD rats was made by four-vessel ligation and given 8g/kg in Huoxue group, 40g/kg in Huangqi group, 48g/kg in Fangfang group, and 20mL in sham group and model group. /kg. Serum and brain homogenates were used to determine SOD by xanthine oxidase method and malondialdehyde was measured by thiobarbituric acid method. Nitric oxide reductase method was used to determine the content of nitric oxide. MAIN OUTCOME MEASURES: Serum, brain superoxide dismutase (SOD), malondialdehyde, and nitric oxide levels in each group. Results: The levels of MDA and NO in serum and brain tissue of the model group increased significantly (P<0.001-0.05), and the SOD content decreased significantly (P<0.05, P<0.01). The total formula group and the disassembled group Huangqi group , blood circulation group?