HPLC法测定风痛定胶囊中青藤碱的含量

• 作 者: 仝武军 李丽 徐莉 张小丽
• 机 构: 兰州军区临潼疗养院,兰州军区临潼疗养院第二疗养区,
• 出 处: 中国疗养医学
• 发表日期: 2009年10期
• 关键词: HPLC 风痛 胶囊 风痛定胶囊 青藤碱 高效液相色谱法 峰面积 阴性样品溶液 对照品溶液 线性关系

目的建立风痛定胶囊中青藤碱含量HPLC测定方法。方法色谱柱为CenturySIL 5 μm C18-BDS分析(4.6mm×250mm,5μm)柱,流动相为甲醇:[(乙二胺:水)(1:300)](62:38),流速为0.5mL/min,检测波长为262nm。结果青藤碱在1.06～10.6μg范围内与峰面积呈良好的线性关系,r=0.9998,平均回收率为93.38%,RSD为1.94%(n=5)。结论此方法准确可靠,简单易行,可用于该制剂的质量控制。

Objective To establish a HPLC method for determination of sinomenine in Fengtongding capsule. Methods The column was a CenturySIL 5 μm C18-BDS column (4.6 mm×250 mm, 5 μm). The mobile phase was methanol:[(ethylenediamine:water)(1:300)](62:38). The flow rate was 0.5 mL. /min, detection wavelength is 262 nm. Results The sinomenine had a good linear relationship with the peak area in the range of 1.06 to 10.6 μg, r=0.9998. The average recovery was 93.38% and the RSD was 1.94% (n=5). Conclusion This method is accurate, reliable, simple and easy to use and can be used for the quality control of the preparation.