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金属纳米颗粒经常被用作纳米猝灭剂构建高效荧光传感平台.在本工作中,以FAM标记的DNA为模板,通过还原Ag+制备得到银纳米颗粒(AgNPs).由于AgNPs与DNA间稳固的结合,模板合成AgNPs呈现出很高的猝灭效率并可用作超猝灭剂以构建生物荧光传感平台.作为一种尝试,模板合成的DNA-AgNPs复合物被用作生物硫醇荧光检测.通过形成S-Ag键,硫醇与AgNPs发生作用并将FAM标记DNA从AgNPs表面取代下来,FAM荧光恢复.由于低的荧光背景信号,该传感器具有高信背比.此外,DNA-AgNPs复合物呈现出很好的稳定性,可应用于复杂的实际样品.为验证其在实际样品中检测生物硫醇的可行性,测定了人类尿样中的硫醇含量,其结果显示人类尿样中的硫醇总含量为229μM到302μM.为验证结果的可靠性,少量Cys被额外加入到尿样中,其回收率经测定为98%～103%.
In this work, silver nanoparticles (AgNPs) were prepared by reduction of Ag + with FAM-labeled DNA as template.As a result of the stable between AgNPs and DNA AgNPs synthesized by the template showed high quenching efficiency and could be used as a super-quencher to build a bioluminescence sensing platform.As an attempt, template-synthesized DNA-AgNPs complexes were used as bio-thiol fluorescence detection The formation of S-Ag bond, thiol and AgNPs role and the FAM labeled DNA replaced from the surface of the AgNPs, FAM fluorescence recovery due to low fluorescence background signal, the sensor has a high signal to background ratio.In addition, DNA-AgNPs complex Exhibits good stability and can be applied to complex real samples.To verify its feasibility of detecting bioschemes in real samples, the thiol content in human urine samples was determined and the results showed that in human urine samples The total thiols ranged from 229 μM to 302 μM To verify the reliability of the results, a small amount of Cys was added to the urine sample with a recovery of 98% to 103%.