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目的:研究甲基阿魏酸(meth-ferulic acid,MFA)在HepG2.2.15细胞中对HBV DNA合成的抑制作用。方法:采用四甲基噻唑蓝(MTT)法检测MFA对HepG2.2.15细胞的半数毒性浓度(TC50)和最大无毒浓度(TC0);在TC0基础上观察5个不同浓度药物作用于HepG2.2.15细胞,分别在第4d和8d收集细胞培养上清液,采用实时荧光定量PCR法检测上清液HBV DNA的含量。结果:TC0=6.6μg/ml,TC50=179.7μg/ml,MFA对HepG2.2.15细胞毒性较低。无毒浓度下的MFA在HepG2.2.15细胞培养中可有效地减少HBV DNA的拷贝数。结论:MFA在HepG2.2.15细胞培养中能有效的抑制HBV DNA的复制,且毒性较低。
AIM: To investigate the inhibitory effect of methy-ferulic acid (MFA) on HBV DNA synthesis in HepG2.2.15 cells. Methods: MTT assay was used to detect the half-value toxicity (TC50) and the maximum non-toxic concentration (TC0) of MFA on HepG2.2.15 cells. Five different concentrations of MFA on HepG2.2.15 cells were observed on the basis of TC0 The cells were collected on the 4th and 8th day respectively, and the supernatant HBV DNA content was detected by real-time fluorescence quantitative PCR. Results: TC0 = 6.6μg / ml, TC50 = 179.7μg / ml, MFA was less cytotoxic to HepG2.2.15. MFA at nontoxic concentrations can effectively reduce HBV DNA copy number in HepG2.2.15 cell culture. Conclusion: MFA can effectively inhibit HBV DNA replication in HepG2.2.15 cell culture with low toxicity.