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通过2-溴-5-碘-3-(三聚氧乙烯)取代噻吩大分子单体的合成、镍催化缩聚等,得到Mn为6000的3-(三聚氧乙烯)取代聚噻吩(P3OET).在非选择性良溶剂四氢呋喃中,P3OET溶液发射橙色荧光.在选择性极性溶剂诱导作用下,P3OET在水中自组装形成稳定的球形自组装体,荧光峰相对红移而发射橙红色光,伴随荧光强度减弱;而在甲醇中,P3OET形成丝状自组装体,荧光峰相对蓝移而发射亮黄色光,但是荧光强度增强,表现出聚集增强发光现象.类似于含有低聚氧乙烯短支链的聚烯烃,P3OET在水溶液中还具有温度响应特性.当水溶液温度升高,荧光峰逐渐蓝移,并且荧光强度增强.P3OET自组装体可进入小鼠黑色素瘤B16F10细胞,并且发光而用于成像.利用控温热台适当加热细胞,或者使用解偶联剂、抗癌药物等化学刺激使细胞活动产生热量时,发光性质随细胞温度升高而发生蓝移、强度增强等规律性变化.
The synthesis of 3- (trimethoxyethylene) substituted polythiophene (P3OET) with an Mn of 6000 was achieved by the synthesis of 2-bromo-5-iodo- 3- (trimethylene oxide) substituted thiophene macromonomer, nickel catalyzed polycondensation, In the non-selective good solvent tetrahydrofuran, P3OET solution emits orange fluorescence.Under the selective polar solvent induction, P3OET self-assemble in water to form a stable spherical self-assembly, the fluorescence peak is relatively red-shifted and emits orange-red light, Accompanied by a decrease in fluorescence intensity; while in methanol, P3OET form filamentous self-assembly, the fluorescence peak relative to the blue shift and emit bright yellow light, but the fluorescence intensity increases, showing aggregation to enhance the luminescence phenomenon similar to containing short branches of polyoxyethylene Chain polyolefins, P3OET also has a temperature response in aqueous solution.When the temperature of the aqueous solution increases, the fluorescence peak gradually blue-shifted, and the fluorescence intensity increased.P3OET self-assembly can enter the mouse melanoma B16F10 cells and emit light for Imaging.Using the temperature control hot stage appropriate heating cells, or use the uncoupling agent, anti-cancer drugs and other chemical stimuli make the cell activity of the heat, the light-emitting properties with the cell temperature rise blue shift, increased intensity and other regular changes.