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目的 建立同时测定贯叶金丝桃中 4种黄酮芦丁、金丝桃苷、扁蓄苷和槲皮素的HPLC分析方法。方法 C18柱 ;流动相A :水 (磷酸调pH 3 1～ 3 5) ,B :乙腈 ,梯度洗脱 ;流速 1 0mL·min- 1;检测波长2 54nm。结果 线性范围为芦丁 1 376～ 8 2 56μg·mL- 1(γ=0 9999) ,金丝桃苷 3 1 60～1 8 960 μg·mL- 1(γ=0 9996) ,扁蓄苷 0 968～ 5 80 8μg·mL- 1(γ =0 9998) ,槲皮素 0 776～ 4 656μg·mL- 1(γ =0 9993)。平均加样回收率为芦丁 97 8% ,RSD(n=3) 4 8% ;金丝桃苷 1 0 0 7% ,RSD(n=3) 4 1 % ;扁蓄苷 97 3% ,RSD(n =3)0 7% ;槲皮素 1 0 0 5 % ,RSD(n =3) 4 4%。 4个化合物的精密度RSD(n =5)均 <2 % ,重现性RSD(n =5)均<3 %。结论 本方法简单、有效、可行 ,可用于贯叶金丝桃黄酮的含量测定
Objective To establish an HPLC method for the simultaneous determination of four kinds of flavonoids rutin, hyperoside, picoside and quercetin in Hypericum perforatum. Methods C18 column; mobile phase A: water (pH adjusted to pH 3 1 - 35), B: acetonitrile, gradient elution; flow rate 10 mL·min - 1; detection wavelength 2 54 nm. The linear range of the results was rutin 1,376 to 8,256 μg·mL-1 (γ=0,9999), hyperoside 3 1,60 to 1,8,960 μg·mL-1 (γ=0,996), and glycoside 0. 968 to 580 80 μg·mL-1 (γ=0 9998), quercetin 0 776 to 4 656 μg·mL-1 (γ=0 9993). The average recovery rate was 97.8% for rutin, 48% for RSD (n=3), 10.7% for hyperoside, 41% for RSD (n=3), and 37% for RS. (n = 3) 0 7%; quercetin 1 0 0 5 %, RSD (n = 3) 4 4%. The RSD (n = 5) and RSD (n = 5) of the four compounds were <2% and <3%, respectively. Conclusion This method is simple, effective and feasible for the determination of flavonoids in Hypericum perforatum.