论文部分内容阅读
为确定多巴胺DA-2受体在人类蜕膜组织中的存在及多巴胺是否能通过DA-2受体促进PGF的产生进行研究。方法:①取3例健康孕妇足月阴道分娩的蜕膜,在冰冷缓冲液中使之成匀浆,多次离心后获得富含膜的部分(P3)用Bio-Rad蛋白分析试剂盒经染料结合分析法来确定受体标本中的蛋白浓度。然后把每例蜕膜分成3份,以[~3H]螺环哌啶酮为DA-2受体的配体,与受体标本在37℃、伴有或不伴有
Studies were conducted to determine the presence of dopamine DA-2 receptor in human decidual tissue and whether dopamine can promote the production of PGF through the DA-2 receptor. Methods: (1) The decidua of full-term vaginal delivery in 3 healthy pregnant women were obtained and homogenized in ice-cold buffer to obtain the membrane-rich fraction after repeated centrifugation (P3). The cells were stained with Bio-Rad Protein Assay Kit The binding assay is used to determine the protein concentration in the recipient sample. Each decidua was then divided into 3 parts, with [~ 3H] spiperone as a DA-2 receptor ligand, with receptor specimens at 37 ° C, with or without