侵染云南花魔芋的马铃薯Y病毒属病毒分离物的检测及分子鉴定

• 作 者: 罗延青 丁铭 方琦 董家红 王玲 李永军 张仲凯
• 机 构: 云南大学生命科学学院,云南省农业科学院生物技术与种质资源研究所,
• 出 处: 植物病理学报
• 发表日期: 2009年01期
• 关键词: 侵染 云南省 花魔芋 马铃薯 病毒分离物 检测 分子鉴定 Yunnan province identification of molecular 生物资源开发产

YN80 was isolated from Amorphophallus rivieri Durieu showing mosaic and crinkle symptoms in Songming,Yunnan province.Flexuous filamentous particles were found in diseased leave sap and pinwheel inclusion bodies were found in the leave tissue.YN80 had positive reaction to universal antibody of Potyvirus by DAS-ELISA.3’-terminal sequence of YN80 was cloned and sequenced.cp gene of YN80 consisted of 987 nt,encoded 328 aa(36.1 kDa).Sequence analysis showed that YN80 shared the highest identity(97.0%)with CP amino acid sequence of Dasheen mosaic virus(DsMV).These data indicated that YN80 was an isolate of DsMV.This is the first molecular identification of A.rivieri Durieu isolate of DsMV in China.

YN80 was isolated from Amorphophallus rivieri Durieu showing mosaic and crinkle symptoms in Songming, Yunnan province. Fs. Aborted found in diseased leave sap and pinwheel inclusion bodies were found in the leave tissue .YN80 had positive reaction to universal antibody of Potyvirus by DAS- ELISA.3’-terminal sequence of YN80 was cloned and sequenced.cp gene of YN80 consisted of 987 nt, encoded 328 aa (36.1 kDa) .Sequence analysis showed that YN80 shared the highest identity (97.0%) with CP amino acid sequence of These data indicated that YN80 was an isolate of DsMV.This is the first molecular identification of A.rivieri Durieu isolate of DsMV in China.