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本试验用 Co60γ射线照射高粱晋粱五号、Tx622B、关东青、同源四倍体。M_2按 M_1结实率四个等级种成穗行,测定 M_1结实率和 M_2各类突变频率的关系,结果表明,M_1生理损伤效应和 M_2突变频率及突变谱有一定的关联性。苗期叶绿素突变频率随着 M_1结实率的提高而增高,呈正相关(r=+0.9783);早熟突变频率随着 M_1结实率的提高而降低,呈负相关(r=-0.70565);总突变频率呈极显著负相关(r=-0.9056);结实率与其他类型突变率无关。通过 M_1和 M_2代经济性状与对照相互比较,表明 M_1结实率和千粒重二者呈正相关,但 M_2代不同结实率级别的千粒重增减程度又不相同。这些结果予示在辐射育种中,选择 M_1结实率较低的穗,可以大大缩小 M_2代种植群体并提高选择效果。
The experiment with Co60γ ray irradiation sorghum Jin Liang Wu, Tx622B, off East Green, autotetraploid. The results showed that there was a correlation between M_1 physiological damage effect and M_2 mutation frequency and mutation spectrum. The frequency of chlorophyll mutation at seedling stage was positively correlated with the increase of M_1 seed setting rate (r = +0.9783). The frequency of precocious mutation decreased with the increase of M_1 seed setting rate (r = -0.70565) There was a significant negative correlation (r = -0.9056). The seed setting rate was not related to other types of mutation rates. Comparing the economic traits of M_1 and M_2 with those of the control, it showed that there was a positive correlation between M_1 seed setting rate and 1000-grain weight, but the increase and decrease of 1000-grain weight at M_2 generation were not the same. These results show that in radiation breeding, the selection of M_1 low spikelet can greatly reduce the M_2 generation colonies and improve the selection effect.