A New Approach to Natural Products Discovery Exemplified by the Identification of Sulfated Nucleosid

• 作 者: Andrew E. Taggi Jerrold Meinwald Frank C. Schroeder
• 机 构: Contribution from The Cornell Institute for Research in Chemical Ecology and The Department of Chemi
• 出 处: 天然产物研究与开发
• 发表日期: 2004年05期

Using a new approach based on the NMR spectroscopic analysis of the entire, unpurified spider venom,we identified a family of unusual sulfated nucleoside derivatives from the venom of the hobo spider,Tegenaria agrestis.These compounds are ribonucleoside mono- and disulfates derived from guanosine and xanthosine, some of which are glycosylated, bearing one or two D-fucose units. The use of NMR spectroscopy to characterize the unfractionated venom was central to the discovery of this heretofore overlooked class of venom components.

Using a new approach based on the NMR spectroscopic analysis of the entire, unpurified spider venom, we identified a family of unusual sulfated nucleoside derivatives from the venom of the hobo spider, Tegenaria agrestis. These compounds are ribonucleoside mono- and disulfates derived from guanosine and xanthosine, some of which are glycosylated, one or two D-fucose units. The use of NMR spectroscopy to characterize the unfractionated venom was central to the discovery of this heretofore overlooked class of venom components.